Journal:

Article Title: Characterization of an Escherichia coli O157:H7 Plasmid O157 Deletion Mutant and Its Survival and Persistence in Cattle ▿

doi: 10.1128/AEM.02643-06

Figure Lengend Snippet: Effect of pO157 on survival of E. coli O157:H7 in synthetic bovine gastric fluid, bile, or low-pH medium. The WT or the ΔpO157 mutant was exposed to each medium for 1 h at 37°C. The number of E. coli cells was determined by direct plate counting on LB agar before and after exposure, and the percent survival was calculated. SGF 3.0, synthetic bovine gastric fluid adjusted to pH 3.0; SGF 2.2, synthetic bovine gastric fluid adjusted to pH 2.2; bile, TSB with 0.15% bovine bile (final concentration); 2.2, TSB adjusted to pH 2.2; bile 2.2, TSB with 0.15% bovine bile (final concentration) adjusted to pH 2.2.

Article Snippet: Three E. coli O157:H7 strains were used in this study: strain ATCC 43894, a human stool isolate producing Shiga toxin types 1 and 2 (referred to as the WT); strain 277, an isogenic strain with the 92-kb plasmid pO157 deleted (referred to as the ΔpO157 mutant); and the complemented strain ΔpO157 mutant 277::pO157Δ ehxA (referred to as the complement) ( 52 ).

Techniques: Mutagenesis, Concentration Assay

Journal:

Article Title: Characterization of an Escherichia coli O157:H7 Plasmid O157 Deletion Mutant and Its Survival and Persistence in Cattle ▿

doi: 10.1128/AEM.02643-06

Figure Lengend Snippet: Effect of pO157 on survival of E. coli O157:H7 in cattle following oral administration of bacteria. Four steers (steers 1 to 4) were given a single oral dose of 1.0 ×1010 CFU containing both the WT and the ΔpO157 mutant. RAMS samples were cultured by direct plating onto SMAC-CTMV, and sorbitol-negative MUG-negative colonies were confirmed to be the O157 serotype by latex agglutination. At least 15 isolates from each sample were subcultured and differentiated as the WT or the ΔpO157 mutant by PCR. Bar heights represent to total number of E. coli O157:H7 isolates recovered from the steer expressed as log CFU/swab. The proportion of isolates that were either the WT (open white bars) or the ΔpO157 mutant (dotted bars) is shown. Asterisks indicate significant differences between the WT and the ΔpO157 mutant (day 1, P < 0.001; day 3, P < 0.05).

Article Snippet: Three E. coli O157:H7 strains were used in this study: strain ATCC 43894, a human stool isolate producing Shiga toxin types 1 and 2 (referred to as the WT); strain 277, an isogenic strain with the 92-kb plasmid pO157 deleted (referred to as the ΔpO157 mutant); and the complemented strain ΔpO157 mutant 277::pO157Δ ehxA (referred to as the complement) ( 52 ).

Techniques: Bacteria, Mutagenesis, Cell Culture, Agglutination

Journal:

Article Title: Characterization of an Escherichia coli O157:H7 Plasmid O157 Deletion Mutant and Its Survival and Persistence in Cattle ▿

doi: 10.1128/AEM.02643-06

Figure Lengend Snippet: E. coli O157:H7 carriage in cattle given a single oral dose of the WT or the ΔpO157 mutant

Article Snippet: Three E. coli O157:H7 strains were used in this study: strain ATCC 43894, a human stool isolate producing Shiga toxin types 1 and 2 (referred to as the WT); strain 277, an isogenic strain with the 92-kb plasmid pO157 deleted (referred to as the ΔpO157 mutant); and the complemented strain ΔpO157 mutant 277::pO157Δ ehxA (referred to as the complement) ( 52 ).

Techniques:

Journal: Frontiers in Microbiology

Article Title: Modeling the Survival of Escherichia coli O157:H7 Under Hydrostatic Pressure, Process Temperature, Time and Allyl Isothiocyanate Stresses in Ground Chicken Meat

doi: 10.3389/fmicb.2018.01871

Figure Lengend Snippet: Logarithmic reductions of E. coli O157:H7 on ground chicken meat after different hydrostatic pressure treatments at 4°C and 15 min (without AITC added).

Article Snippet: Three E. coli O157:H7 (strains: C9490, 59762 and 59768) available and purchased from the American Type Culture Collection (ATCC, Manassas, VA, United States) were used.

Techniques:

Journal: Frontiers in Microbiology

Article Title: Modeling the Survival of Escherichia coli O157:H7 Under Hydrostatic Pressure, Process Temperature, Time and Allyl Isothiocyanate Stresses in Ground Chicken Meat

doi: 10.3389/fmicb.2018.01871

Figure Lengend Snippet: Reductions of Escherichia coli O157:H7 on ground chicken meat after different AITC concentration (%) treated for 24 h.

Article Snippet: Three E. coli O157:H7 (strains: C9490, 59762 and 59768) available and purchased from the American Type Culture Collection (ATCC, Manassas, VA, United States) were used.

Techniques: Concentration Assay

Journal: Frontiers in Microbiology

Article Title: Modeling the Survival of Escherichia coli O157:H7 Under Hydrostatic Pressure, Process Temperature, Time and Allyl Isothiocyanate Stresses in Ground Chicken Meat

doi: 10.3389/fmicb.2018.01871

Figure Lengend Snippet: Logarithmic reductions of Escherichia coli O157:H7 on ground chicken meat after different high pressure level treatments at different operation temperatures for 15 min. The initial inoculum count of the E. coli O157:H7 was at 8.0 log CFU/g level. The detection limit was 1.0 log CFU/g. Results are shown as mean ± standard deviation ( N = 3, with 2 petrifilm counts per run; n = 2 × 3 random runs).

Article Snippet: Three E. coli O157:H7 (strains: C9490, 59762 and 59768) available and purchased from the American Type Culture Collection (ATCC, Manassas, VA, United States) were used.

Techniques: Standard Deviation

Journal: Frontiers in Microbiology

Article Title: Modeling the Survival of Escherichia coli O157:H7 Under Hydrostatic Pressure, Process Temperature, Time and Allyl Isothiocyanate Stresses in Ground Chicken Meat

doi: 10.3389/fmicb.2018.01871

Figure Lengend Snippet: Logarithmic reductions of E. coli O157:H7 on ground chicken meat after high pressure processing treatments according to the four-parameter, two-level factorial design.

Article Snippet: Three E. coli O157:H7 (strains: C9490, 59762 and 59768) available and purchased from the American Type Culture Collection (ATCC, Manassas, VA, United States) were used.

Techniques: Concentration Assay

Journal: Frontiers in Microbiology

Article Title: Modeling the Survival of Escherichia coli O157:H7 Under Hydrostatic Pressure, Process Temperature, Time and Allyl Isothiocyanate Stresses in Ground Chicken Meat

doi: 10.3389/fmicb.2018.01871

Figure Lengend Snippet: Verification of predictive models (Eq. 2 and Eq. 3) for log reduction of E. coli O157:H7 in raw ground chicken meat.

Article Snippet: Three E. coli O157:H7 (strains: C9490, 59762 and 59768) available and purchased from the American Type Culture Collection (ATCC, Manassas, VA, United States) were used.

Techniques: Concentration Assay

Journal: Frontiers in Microbiology

Article Title: Modeling the Survival of Escherichia coli O157:H7 Under Hydrostatic Pressure, Process Temperature, Time and Allyl Isothiocyanate Stresses in Ground Chicken Meat

doi: 10.3389/fmicb.2018.01871

Figure Lengend Snippet: The survival behaviors of E. coli O157:H7 stored at 4 and 10°C, for 9–10 days after HPP treatment with conditions: 300 MPa, 15 min, 4°C and 0.12% AITC concentration (%). The initial inoculum counts of the E. coli O157:H7 was at 8.0 log CFU/g level.

Article Snippet: Three E. coli O157:H7 (strains: C9490, 59762 and 59768) available and purchased from the American Type Culture Collection (ATCC, Manassas, VA, United States) were used.

Techniques: Concentration Assay